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The F1 generation demonstrates 45% sperm count reduction and 38% testosterone decrease, while the F2 generation exhibits persistent sperm motility and morphological abnormalities attributable to heritable DNA methylation changes

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Generation of α 2A -adrenergic receptor knockout cell line We employed a CRISPR-Cas9-mediated approach to delete endogenous α 2A -adrenergic receptor expression in INS-1E cells