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Given our observation that the peak of daily GFAT activity rhythm is always fixed right after the natural feeding time window in WT flies, even when flies were fed at unnatural feeding time (18), we concluded that circadian clocks impose a stronger regulation on GFAT activity rhythm as compared to nutrient input (Fig

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The cells were thawed and cultured in StemSpan SFEM II culture medium, supplemented with 1X CC100 (containing the cytokines FLT3L, SCF, IL3, and IL6), recombinant thrombopoietin (TPO) (100ng/ml), and UM171 (35nM), as we have described previously 29 Xenotransplantation and animal models All animal procedures were performed under the protocol approved by Boston Children’s Hospital Institutional Animal Care and Use Committee (IACUC)